Metamitron Aptamer (MTM-6) Aptamer Details

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Metamitron Aptamer (MTM-6) (ID# 10064)

Aptamer Chemistry: DNA

Target: Metamitron

Antigen/Target Category: Small Organic

Affinity (Kd): 16 nM (reported value)

Binding Conditions/Buffer: Tris-acetate EDTA (TAE) buffer, pH 8.3

Binding Temp: 25 °C°C

Refolding Protocol: Heated at 95C for denaturation for 6 minutes, cooled in ice for 10 minutes, and left at 25C for 5 minutes..

Specificity: Selective against several frequently and widely used pesticides including pentachloro-nitrobenzene (PCNB), fipronil (FIP), procymidone (PROC), thiazolidinediones (TZD), cyfluthrin (CF), pyridaben (PYR), metalaxyl (MET), iprodione (IPRO), and butachlor. (BUT)

Sequence ():

Length: 60 Base Pairs

Molecular Weight: 18442.9 g/mole

Extinction Coefficient: 544400 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Xicheng Xie, Weiwen Huang, Guoqing Shen, Hong Yu, Lumei Wang; Selection and colorimetric application of ssDNA aptamers against metamitron based on magnetic bead-SELEX. Anal. Methods 2022; 14 (31): 3021–3032. https://doi.org/10.1039/d2ay00566b

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