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Apta-Index™: 2F11-14 (ID# 581)

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2F11-14 (ID# 581)

2F11-14
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA Ebola virus inhibitory domain (eVP35 IID) (EBOV) (eVP35) Protein 7.1 nM (reported value) 10mM HEPES (pH 7.0), 150mM NaCl, 2mM TCEP (tris(2-carboxyethyl)phosphine), 1mM MgCl2 N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
rGprGprGprAprGprAprCprAprAprGprAprApUrAprAprAprCprGprCpUrCprAprAprCprGpUUrCprAprGpUrApUrAprAprCprAprGpUrCprCprGprAprGpUrCpUrAprAprCprAprCprAprCprAprApUrGprGprGprAprCprAprCpUrGprAprApUUrCprGprAprCprAprGprGprAprGprGprCpUrCprAprCprAprAprCprAprGprGprCp
78 25439.76 g/mole 797500.00 L/(mole·cm) 56.41% 1.25 31.90

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Jennifer M. Binning, Tianjiao Wang, Priya Luthra, Reed S. Shabman, Dominika M. Borek, Gai Liu, Wei Xu, Daisy W. Leung, Christopher F. Basler, and Gaya K. Amarasinghe, “Development of RNA aptamers targeting ebolavirus VP35” Biochemistry 2013, 52, 8406-8419.

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