Aptagen, LLC
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
2'-F-RNA
Protein E (PE)
Protein
1.2 nM (reported value)
165 ug (~5 nmol) purified truncated His-tagged PE immobilized on Niƒ??NTA agarose (Qiagen) in 1.6 mL PBSM (PBS with 1 mM MgCl2)
23°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Clone 2 (ID# 547)
221
20369.18 g/mole
643700 L/(mole·cm)
68.18%
1.55
31.64
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Barfod, Anders et al. "In Vitro Selection of RNA Aptamers Directed Against Protein E: A Haemophilus influenzae Adhesin." Mol Biotech, 2014: 1-12.
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