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Apta-Index™: Clone 2 (ID# 547)

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Clone 2 (ID# 547)

Clone 2
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
2'-F-RNA Protein E (PE) Protein 1.2 nM (reported value) 165 ug (~5 nmol) purified truncated His-tagged PE immobilized on Ni–NTA agarose (Qiagen) in 1.6 mL PBSM (PBS with 1 mM MgCl2) N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
dCpdGpdApdCpUdGpdCpdApdGpdApdGpdCpUUdGpdCpUdApdCpdGpUUdGpUdApdApUUdCpdApdApdApdApdCpdApdApdApdGpdGpUUUdCpdCpUdGpdGpdApdCpUdCpdCpdCpdCpdGpdCpdApdGpdGpUdCpUUdGpUdGpdApdApUdGpdGpUdApdCpdCpdGpdApdGpdCpUdCpdGpdApdApUUdCpdCpdCp
66 20369.18 g/mole 643700.00 L/(mole·cm) 68.18% 1.55 31.64

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Barfod, Anders et al. "In Vitro Selection of RNA Aptamers Directed Against Protein E: A Haemophilus influenzae Adhesin." Mol Biotech, 2014: 1-12.

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