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Apta-Index™: Mycoplasma bovis detection aptamer (WKB-14) (ID# 544)

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Mycoplasma bovis detection aptamer (WKB-14) (ID# 544)

Mycoplasma bovis detection aptamer (WKB-14)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA P48 of M. Bovis Protein 15.61 nM (reported value) Added to wells of plate coated with P48 protein solution (P48 protein dissolved in 100 uL of 0.05 carbonate-bicarbonate buffer) N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
dGpdCpdTpdGpdCpdApdApdTpdApdCpdTpdCpdApdTpdGpdGpdApdCpdApdGpdGpdTpdTpdGpdCpdGpdApdApdApdGpdApdCpdApdApdCpdGpdApdApdTpdGpdCpdTpdTpdTpdGpdCpdCpdTpdGpdCpdCpdApdTpdApdApdTpdTpdTpdGpdCpdGpdTpdCpdTpdGpdGpdApdGpdTpdApdCpdGpdApdCpdCpdCpdTpdGpdApdAp
80 24687.08 g/mole 769800.00 L/(mole·cm) 48.75% 1.30 32.07

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Fu, P., et. al. Enzyme linked aptamer assay: based on a competition format for sensitive detection of antibodies to Mycoplasma bovis in serum.

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