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Apta-Index™: Nanobioprobe mediated DNA aptamer (ID# 539)

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Nanobioprobe mediated DNA aptamer (ID# 539)

Nanobioprobe mediated DNA aptamer
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Trinitrotoluene (TNT) Other 100 nM (reported value) 10 mM Tris/HCl buffer, pH 7.6, 150 mM NaCl, 5 mM MgCl2 N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. The 5' end of the designed primer was labeled with FITC and thiol (-SHC6) to monitorthe progression of selection rounds and the binding of aptamers on a gold substrate respectively. The 3' end of the designed primer was labeled with biotin.
dApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdGpdGpdGpdApdCpdApdGpdTpdCpdGpdApdTpdGpdGpdGpdApdCpdGpdGpdCpdApdApdApdCpdGpdGpdApdCpdCpdApdGpdTpdGpdTpdGpdTpdGpdGpdCpdGpdGpdTpdApdGpdApdTpdApdGpdTpdApdApdGpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp
88 27415.90 g/mole 890400.00 L/(mole·cm) 46.59% 1.12 30.79

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Priyanka, M. S., et al. "Nanobioprobe mediated DNA aptamers for explosive detection." Chem. Commun., 2014, 50, 1080 - 1082.

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