Aptagen, LLC
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
2'-NH2-RNA
Interferon-y/IFN-y/IFNG
Protein
1.7 nM (reported value)
Binding Mixture: PBS (138 mM NaC1, 2.7 mM KCl, 8.1 mM Na2HPO4, and 1.1 mM KH2PO4, pH 7.4) containing 1 mM Mg2+ ions and 0.01% human serum albumin.
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
This is a biphasic binding aptamer described by a two-Kd model, 1.4/75 nM. No inhibition of IFNG binding to its receptor was observed
The highlighted region in the structure represents the minimal effective sequence. However, the sequence could not be transcribed and no binding information was available.
Interferon-γ (2'NH2-17) (ID# 479)
The highlighted region in the structure represents the minimal effective sequence. However, the sequence could not be transcribed and no binding information was available.
5'rGprGprGprAprGprGprApnCprGprApnUprGpnCprGprGp nUprGprGp nUprAprGp nCprGpnCp rGprApnUp rApnUprAp rGpnCprGp nCpnUprGp rGpnUprAp rGprGprGp nUpnUprGp nCpnCprGp rGpnUprGp rApnUp nCprAprGprApnCprGprApnCpnUpnCprGpnCpnCpnCprGprAp 3'
75
38504.53 g/mole
682800 L/(mole·cm)
63.77%
1.46
56.39
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Kubik, Mark F., et al. "Isolation and Characterization of 2ƒ??-Fluoro-, 2ƒ??-Amino-, and 2ƒ??-Fluoro-/Amino-Modified RNA Ligands to Human IFN-7 That Inhibit Receptor Binding." Journal of Immunology, 159 (1997): 259-267.
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