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Apta-Index™: Pro-Gastrin Releasing Peptide (ID# 471)

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Pro-Gastrin Releasing Peptide (ID# 471)

Pro-Gastrin Releasing Peptide
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Pro-gastrin releasing peptide (proGRP) Protein 1.2 µM (reported value) SELEX buffer used in SPR binding assay: 137 mM NaCl, 8.1 mM Na2HPO4, 2.68 mM KCl, 1.47 mM KH2PO4, 0.9 mM CaCl2, 5.3 mM MgCl2, and 4.5 g/L glucose (pH 7.4) N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-dApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTp dCpdApdApdTpdTpdTpdGpdCpdApdCpdCpdApdCpdTpdTpdApdTpdTpdGpdTpdTpdApdCpdTpdApdApdTpdCpdTpdGpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp-3'
61 18685.27 g/mole 593700.00 L/(mole·cm) 32.79% 1.68 31.47

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

M Mie et al. (2012) Selection of DNA aptamers with affinity for Pro-gastrin-Releasing Peptide (proGRP), a tumor marker for small cell lung cancer. Appl. Biochem. Biotechnol. Published Online ahead of Print: DOI 10.1007/s12010-012-9956-5

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