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Apta-Index™: Acetylcholine (ACh 6R) (ID# 442)

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Acetylcholine (ACh 6R) (ID# 442)

Acetylcholine (ACh 6R)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Acetylcholine Small Organic N/A nM (reported value) Binding Buffer: 0.5 M NaCl, 10 mM Tris HCl, and 1 mM MgCl2.
Incubation time: 1 hour
N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-dApdTpdCpdCpdGpdTpdCpdApdCpdApdCpdCpdTpdGpdCpdTpdCpdTpdCpdApdGpdGpdGpdGpdApdTpdCpdApdCpdApdTpdTpdCpdTpdTpdGpdApdCpdGpdGpdTpdGpdTpdGpdApdTpdApdCpdApdGpdTpdGpdCpdCpdTpdGpdGpdTpdGpdTpdTpdGpdGpdCpdTpdCpdCpdCpdGpdTpdApdTp-3'
72 22123.35 g/mole 666100.00 L/(mole·cm) 54.17% 1.50 33.21

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

J.G. Bruno et al. Development of DNA aptamers for cytochemical detection of acetylcholine. In Vitro Cell. Dev. Biol.-Anim. 44(2008):63-72.

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