Aptagen, LLC
DNA
HIV RT
Protein
80 nM (reported value)
50 mM Tris-HCl, pH 8, 1mM DTT, 80 mM KCl, 6 mM MgCl2 for ten minutes.
RT°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
HIV Reverse Transcriptase (PF1) (ID# 416)
5'dApdGpdGpdApdApdGpdGpdCpdTpdTp dTpdApdGpdGpdTpdCpdTpdGpdApdGp dApdTpdCpdTpdCpdGpdGpdApdApdTp3'
30
9326.14 g/mole
300100 L/(mole·cm)
46.67%
3.33
31.08
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Y-T Lai and J DeStefano. DNA aptamers to human immunodeficiency virus reverse transcriptase selected by a primer-free SELEX method: characterization and comparison with other aptamers. Nucleic Acid Therapeutics 22(2012): 162-176.
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