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Apta-Index™: HIV Reverse Transcriptase (PF1) (ID# 416)

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HIV Reverse Transcriptase (PF1) (ID# 416)

HIV Reverse Transcriptase (PF1)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA HIV RT Protein 80 nM (reported value) 50 mM Tris-HCl, pH 8, 1mM DTT, 80 mM KCl, 6 mM MgCl2 for ten minutes. N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-dApdGpdGpdApdApdGpdGpdCpdTpdTp dTpdApdGpdGpdTpdCpdTpdGpdApdGp dApdTpdCpdTpdCpdGpdGpdApdApdTp-3'
30 9326.14 g/mole 300100.00 L/(mole·cm) 46.67% 3.33 31.08

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Y-T Lai and J DeStefano. DNA aptamers to human immunodeficiency virus reverse transcriptase selected by a primer-free SELEX method: characterization and comparison with other aptamers. Nucleic Acid Therapeutics 22(2012): 162-176.

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