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Apta-Index™: Vaspin binding aptamer (VBA) (ID# 409)

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Vaspin binding aptamer (VBA) (ID# 409)

Vaspin binding aptamer (VBA)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Vaspin Protein 303 nM (reported value) Binding Buffer: 100 mM NaCl, 25 mM Tris-HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, and 0.02% Tween-20, pH 7.6. N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Limit of detection using ELAAS assay was 39 ng/ml. See SJ Lee et al (2012) for more details.
5'-dApdTpdAp dCpdCpdAp dGpdCpdTp dTpdApdTp dTpdCpdAp dApdTpdTp dGpdGpdGp dCpdGpdGp dTpdGpdGp dGpdGpdGp dGpdGpdGp dTpdApdGp dTpdGpdGp dGpdTpdGp dTpdTpdAp dTpdGpdGp dCpdGpdAp dTpdCpdGp dTpdGpdGp dApdGpdAp dTpdApdGp dTpdApdAp dGpdTpdGp dCpdApdAp dTpdCpdTp-3'
75 23487.31 g/mole 743100.00 L/(mole·cm) 50.67% 1.35 31.61

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

SJ Lee et al. Sensitive detection of adipokines for early diagnosis of type 2 diabetes using enzyme-linked antibody-aptamer sandwich (ELAAS) assays. Sensors Actuat. B-Chem.(2012): 243-248.

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