Aptagen, LLC
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
2'-F-RNA
Interferon-y/IFN-y/IFNG
Protein
4.4 nM (reported value)
Binding Mixture: PBS (138 mM NaC1, 2.7 mM KCl, 8.1 mM Na2HPO4, and 1.1 mM KH2PO4, pH 7.4) containing 1 mM Mg2+ ions and 0.01% human serum albumin.
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
This is a biphasic binding described by a two-Kd model, 4.4/716 nM.
Interferon-γ (2'F-1) (ID# 406)
5'rGprGprGprAprGprGprApfCprGprApfUprGpfCprGprGprApfCprApfCpfCprGpfUpfUprAprApfUpfCpfUprGprAprGprGpfCpfCpfCpfUprGpfUpfCpfCpfUprApfUpfUpfCpfCpfUpfUpfCprApfCprGpfCpfCpfUpfCprAprGprAp3'
59
18947.24 g/mole
566400 L/(mole·cm)
57.63%
1.77
33.45
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Kubik, Mark F., et al. "Isolation and Characterization of 2ƒ??-Fluoro-, 2ƒ??-Amino-, and 2ƒ??-Fluoro-/Amino-Modified RNA Ligands to Human IFN-7 That Inhibit Receptor Binding." Journal of Immunology, 159 (1997): 259-267.
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