Aptagen, LLC
RNA
Dopamine
Small Organic
1 pM (reported value)
Assay Buffer: 10 mM PBS, pH 7.2, 5mM MgCl2
RT°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Cross-reactivity was performed on 3-methoxytyramine, epinephrine, homovanillic acid, 3,4-dihydroxyphenylacetic acid (cross-reactivity less than 1% for these reactants) and norepinephrine (3.68%).
Dopamine (dopa2/c.4) (ID# 401)
5'rGprGprGp rAprAprUp rUprCprCp rGprCprGp rUprGprUp rGprCprGp rCprCprGp rCprGprGp rAprAprGp rAprGprGp rGprAprAp rUprAprUp rAprGprAp rGprGprCp rCprAprGp rCprAprCp rAprUprAp rGprUprGp rAprGprGp rCprCprCp rUprCprCp rUprCprCp rCp3'
74
21676.14 g/mole
652400 L/(mole·cm)
62.69%
1.53
33.23
Sequence from C Mannironi, et al. "In Vitro Selection of Dopamine RNA Ligands." Biochemistry, 36 (1997): 9726-973.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Sequence from C Mannironi, et al. "In Vitro Selection of Dopamine RNA Ligands." Biochemistry, 36 (1997): 9726-973.
Enzyme-linked assay from H Park and I R Paeng. "Development of direct competitive enzyme-linked aptamer assay for determination of dopamine in serum." Analytica Chimica Acta, 685 (2011): 65-73.
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