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Apta-Index™: Dopamine (dopa2/c.4) (ID# 401)

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Dopamine (dopa2/c.4) (ID# 401)

Dopamine (dopa2/c.4)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA Dopamine Small Organic 2.8 µM (reported value) Assay Buffer: 10 mM PBS, pH 7.2, 5mM MgCl2 N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-rGprGprGp rAprAprUp rUprCprCp rGprCprGp rUprGprUp rGprCprGp rCprCprGp rCprGprGp rAprAprGp rAprGprGp rGprAprAp rUprAprUp rAprGprAp rGprGprCp rCprAprGp rCprAprCp rAprUprAp rGprUprGp rAprGprGp rCprCprCp rUprCprCp rUprCprCp rCp-3'
67 21676.14 g/mole 652400.00 L/(mole·cm) 62.69% 1.53 33.23

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Sequence from C Mannironi, et al. "In Vitro Selection of Dopamine RNA Ligands." Biochemistry, 36 (1997): 9726-973.
Enzyme-linked assay from H Park and I R Paeng. "Development of direct competitive enzyme-linked aptamer assay for determination of dopamine in serum." Analytica Chimica Acta, 685 (2011): 65-73.

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