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Apta-Index™: theophylline-inhibited molecular switch (ID# 398)

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theophylline-inhibited molecular switch (ID# 398)

theophylline-inhibited molecular switch
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA theophylline Small Organic na nM (reported value) Reaction buffer [50 mM Tris.HCl (pH 7.5) and 20 mM MgCl2] for 20 hr N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-rGprGprGprCprGprAprCprCprCprUprGprAprUprGprAprGprAprUprGp rAprUprAprCprCprAprGprCprCprGprAprAprAprGprGprCprCprCprUprUprGprGprCprAprGp rCprUprCprUprCprGprAprAprAprCprGprGprUprGprAprAprAprGprCprCprGprUprAprGprGprUprUprGprCprCprCp-3'
75 24231.68 g/mole 727900.00 L/(mole·cm) 60.00% 1.37 33.29

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Garrett A. Soukup and Ronald R. Breaker. "Engineering precision RNA molecular switches" Proc. Natl. Acad. Sci. 96(1999):3584-3589.

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