Aptagen, LLC
RNA
NF-kB p65
Protein
25 nM (reported value)
Selection buffer: 10mM HEPES at pH 7.5, 100mM NaCl, 1mM DTT
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Anti-NF-kB p65 (R2) (ID# 388)
5'rGprAprAprGprCprUprUprUprCprAprCprAprCprAprAprCprAprAprGprGprCprCprCprGprGprGprAprCprUprGprUprAprUprUprAprGprGprGprAprAprAprUprUprAprGprAprGprUprAprCprAprGprAprCprAprGprUprCprGprCprCprGprUprGprGprGprUprCprGprAprAprUprUprCprCprGprCprUprCprAp3'
80
25801.63 g/mole
803700 L/(mole·cm)
51.25%
1.24
32.10
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Wurster, S. E, and Maher, L. J. "Selection and characterization of anti-NF-kB p65 aptamers." RNA, 14(2008): 1037-1047.
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