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Apta-Index™: Theophylline + Flavin Mononucleotide (AR7) (ID# 385)

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Theophylline + Flavin Mononucleotide (AR7) (ID# 385)

Theophylline + Flavin Mononucleotide (AR7)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA Theophylline and FMN Protein N/A nM (reported value) Standard Reaction Conditions: 50 mM Tris-HCl, pH 7.5, 20 mM MgCl2
2X Gel Loading Buffer (equal volume used to quench reaction): 87 mM Tris base, 89 mM boric acid, 20% sucrose, 0.05% bromophenol blue, 0.05% xylene cyanol, 0.1% sodium dodecyl sulfate (SDS), 7.3 M urea, 1 mM EDTA. Additional 40 mM EDTA added.
N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Cleaves only in the presence of both theophylline and FMN.
5'-rGprGprAprUprAprAprUprAprGprCprCprGprUprAprGprGprUprUprGprCprGprAprAprAprGprCprGprAprCprCprCprUprGprAprUprGprAprGp rCprCprUprUprAprGprGprAprUprAprUprGprUprCprUprGprGprAprUprAprCprCprAprUprGprCprAprUprGprAprUprGprCprAprCprCprUprUprGprGprCprAprGprUprCprUprUprAprCprAprGprAprAprGprGprAprCprGprUp rCprGprAprAprAprCprGprGprUprAprGprCprGprAprGprAprGprCprUprCp -3'
117 37838.86 g/mole 1175900.00 L/(mole·cm) 51.28% 0.85 32.18

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Seetharaman et al. "Immobilized RNA switches for the analysis of complex chemical and biological mixture." Nature Biotechnology, 19(2001):336-341.

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