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Apta-Index™: Epithelial cell adhesion molecule (EpCAM) (Truncated Clone D) (ID# 373)

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Epithelial cell adhesion molecule (EpCAM) (Truncated Clone D) (ID# 373)

Epithelial cell adhesion molecule (EpCAM) (Truncated Clone D)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA Cancer stem cell marker epithelial cell adhesion molecule Protein 12 nM (reported value) Assay Buffer: DPBS with 5 mM MgCl2, 0.1 mg/ml tRNA, 0.1 mg/ml salmon sperm DNA, 02% [w/v] sodium azide, and 5% FCS. The final concentration of MgCl2 was 2.5 mM. N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Kd values listed for the different cell lines are for the modified version of this aptamer (EpDT3).
5'-rGprCprGprAprCprUprGprGprUprUp rAprCprCprCprGprGprUprCprGp-3'
19 6068.69 g/mole 178500.00 L/(mole·cm) 68.42% 5.60 34.00

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

S Shigdar et al. RNA aptamer against a cancer stem cell marker epithelial cell adhesion molecule. Cancer Sci 102(2011): 991-998.

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