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Apta-Index™: Membrane-Bound IgM (TD05.17) (ID# 351)

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Membrane-Bound IgM (TD05.17) (ID# 351)

Membrane-Bound IgM (TD05.17)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
Chimeric Membrane-bound IgM (mIgM )/ B-cell receptor (BCR )/ Leukemia / Lymphoma Protein 43 nM (reported value) Binding Buffer: RPMI 1640, 4.5 g/L glucose, 5 mM MgCl2 in vivo (20 mM MgCl2 in vitro), 100 mg/L tRNA and 100 mg/L BSA. N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. The monomer does not bind at 37°C (Kd > 10,000 nM). Aptamer was trimerized using PEG linkers. This increased the stability and binding affinity of the aptamer at higher temperatures (Kd = 256 nM at 37°C / Kd = 7.9).

This aptamer is a truncated version of TD05 below.

The four nucleotides at the 3'-tail were replaced with linked nucleic acids to strengthen the stem.
5'-dApdGpdGpdApdGpdGpdApdTpdApdGp dTpdTpdCpdGpdGpdTpdGpdGpdCpdTp dGpdTpdTpdCpdApdGpdGpdGpdTpdCp dTpdCpdCpbTnbCnbCnbTn-3'
37 10256.70 g/mole 349100.00 L/(mole·cm) 56.76% 2.86 29.38

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

R Mallikaratchy et al. "A multivalent DNA aptamer specific for the B-cell receptor on human lymphoma and leukemia." Nucleic Acids Res. 39(2011): 2458-2469.

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