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Apta-Index™: HMBA (aptabeacon) (ID# 334)

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HMBA (aptabeacon) (ID# 334)

HMBA (aptabeacon)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA 6X His-tag Protein ~4.2 nM (reported value) 1X DPBS without Ca and Mg (pH 7.1-7.5), 20 °C N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Aptabeacon specific for proteins containing a terminal His-tag.
5'-{5'/5-FAM}-dCpdApdGpdGpdTpdTpdGpdGpdTpdCpdTpdGpdGpdTpdTpdGpdGpdGpdTpdTpdTpdGpdGpdCpdTpdCpdCpdTpdGpdTpdGpdTpdApdCpdGp-{Internal/Spacer 18}-{Internal/Spacer 18}-{Internal/Spacer 18}-{Internal/Spacer 18}-{Internal/Spacer 18}-{Internal/Spacer 18}-dCpdApdApdCpdCpdTpdGp-{3'/Dabcyl CPG}-3'
42 12990.45 g/mole 382400.00 L/(mole·cm) 57.14% 2.62 33.97

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

X Tan et al. Molecular beacon aptamers for direct and universal quantitation of recombinant proteins from cell lysates. Anal. Chem. [published online ahead of print August 23, 2012]. doi: 10.1021/ac301764q

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