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Apta-Index™: Adipose Tissue (adipo-8) (ID# 326)

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Adipose Tissue (adipo-8) (ID# 326)

Adipose Tissue (adipo-8)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Mature 3T3-L1 adipocytes (adipose tissue) Cells 17.8 nM (reported value) 4.5 mg/ml glucose and 5 mM MgCl2 in D-PBS with calcium chloride and magnesium chloride (Sigma), yeast tRNA (0.1 mg/ml) and BSA (1 mg/ml). pH 7.1-7.5. 4°C N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Aptamer binds to adipose cells at 4 °C and 37°C. Trypsin assay suggests that aptamer binds to a surface protein.
5'-dApdTpdGpdApdGpdApdApdGpdCpdGpdTpdCpdGpdGpdTpdGpdTpdGpdGpdTpdTpdApdApdAp dCpdApdCpdGpdGpdApdApdCpdGpdApdApdGpdGpdTpdGpdCpdApdGpdGpdApdApdGpdApdTpdTpdTpdGpdTpdCpdGpdApdTpdGpdCpdGpdGpdTpdGpdCpdCpdTpdGpdApdGpdCpdGpdGpdGpdCpdTpdGpdGpdCpdApdApdGpdGpdCpdGpdCpdApdTpdAp-3'
87 27226.70 g/mole 862800.00 L/(mole·cm) 56.32% 1.16 31.56

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

J. Liu et. al. "Selection of aptamers specific for adipose tissue." PLoS One, 7(2012): e37789.

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