Aptagen, LLC
DNA
HPV-nontumorigenic HF cell line
Cells
7.1 nM (reported value)
1% BSA in PBS with salts
4°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Aptamer able to penetrate cell surface and internalized in cell cytoplasm independent of protein binding.
HPV-HF cell line (14) (ID# 316)
5'dApdTpdAp dCpdCpdAp dGpdCpdTp dTpdApdTp dTpdCpdAp dApdTpdTp dGpdGpdGp dCpdGpdGp dGpdGpdAp dGpdTpdAp dGpdGpdGp dApdGpdAp dGpdGpdGp dGpdTpdTp dTpdCpdCp dApdTpdCp dGpdGpdCp dGpdApdCp dApdGpdAp dGpdGpdAp dGpdTpdTp dApdTpdGp dTpdGpdTp dGpdTpdAp dGpdApdTp dApdGpdTp dApdApdGp dTpdGpdCp dApdApdTp dCpdTp3'
98
27814.14 g/mole
893700 L/(mole·cm)
48.31%
1.12
31.12
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Graham JC, Zarbl H (2012) Use of Cell-SELEX to Generate DNA Aptamers as Molecular Probes of HPV-Associated Cervical Cancer Cells. PLoS ONE 7(4): e36103. doi:10.1371/journal.pone.0036103
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