Aptagen, LLC
DNA
VEGF (165)
Protein
0.5 nM (reported value)
PBS and 0.005% tween-20 solution mixture was used as the running buffer, and 50 mM NaOH as the regeneration buffer.
25°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
VEGF (SL2-B aptamer) (ID# 303)
5'dCpdApdApdTpdTpdGpdGpdGpdCpdCpdCpdGpdTpdCpdCpdGpdTpdApdTpdGpdGpdTpdGpdGpdGpdTp3'
26
8034.25 g/mole
242600 L/(mole·cm)
61.54%
4.12
33.12
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Kaur H, Yung L-YL (2012) Probing High Affinity Sequences of DNA Aptamer against VEGF165. PLoS ONE 7(2): e31196. doi:10.1371/journal.pone.0031196
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