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Apta-Index™: VEGF (SL2-B aptamer) (ID# 303)

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VEGF (SL2-B aptamer) (ID# 303)

VEGF (SL2-B aptamer)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA VEGF (165) Protein 0.5 nM (reported value) PBS and 0.005% tween-20 solution mixture was used as the running buffer, and 50 mM NaOH as the regeneration buffer. N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-dCpdApdApdTpdTpdGpdGpdGpdCpdCpdCpdGpdTpdCpdCpdGpdTpdApdTpdGpdGpdTpdGpdGpdGpdTp-3'
26 8034.25 g/mole 242600.00 L/(mole·cm) 61.54% 4.12 33.12

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Kaur H, Yung L-YL (2012) Probing High Affinity Sequences of DNA Aptamer against VEGF165. PLoS ONE 7(2): e31196. doi:10.1371/journal.pone.0031196

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