Aptagen, LLC
DNA
Phorate
Small Organic
1.43 µM (reported value)
Selection buffer used in binding affinity assays: 300mM NaCl, 50 mM KCl, 10 mM MgCl2, 50 mM Tris/HCl, and pH 8.3
RT°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
This aptamer binds preferentially to the organophosphates phorate, profenofos, benzoate and omethoate. It has reduced binding to imidacloprid, acetamiprid, chlorpyrifos, methamidophos, dichlorvos, parathion, dimethoate, monocrotophos and phoxim.
"A molecular beacon (5' FAM-CTGCACAAGAATCGCTGCAG-3') was designed, which was partly complementary with the middle constant nucleotides-sequence of the selected ssDNAs."
Phorate (SS4-54) (ID# 296)
5'dApdApdGpdCpdTpdTpdTpdTpdTpdTpdGpdApdCpdTpdGpdApdCpdTpdGpdCpdApdGpdCpdGpdApdTpdTpdCpdTpdTpdGpdApdTpdCpdGpdCpdCpdApdCpdGpdGpdTpdCpdTpdGpdGpdApdApdApdApdApdGpdApdGp3'
54
16675.9 g/mole
517600 L/(mole·cm)
46.30%
1.93
32.22
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Wang, L, et al. (2012). Selection of DNA aptamers that bind four organophosphorus pesticides. Biotechnol Lett, 2012(34), 869-874. doi: 10.1007/s10529-012-0850-6
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