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Apta-Index™: Isocarbophos (SS2-55) (ID# 294)

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Isocarbophos (SS2-55) (ID# 294)

Isocarbophos (SS2-55)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Isocarbophos Small Organic 0.83 µM (reported value) Selection buffer used in binding affinity assays: 300mM NaCl, 50 mM KCl, 10 mM MgCl2, 50 mM Tris/HCl, and pH 8.3 N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. "A molecular beacon (5' FAM-CTGCACAAGAATCGCTGCAG-3') was designed, which was partly complementary with the middle constant nucleotides-sequence of the selected ssDNAs."
5'-dApdApdGpdCpdTpdTpdGpdCpdTpdTpdTpdApdTpdApdGpdCpdCpdTpdGpdCpdApdGpdCpdGpdApdTpdTpdCpdTpdTpdGpdApdTpdCpdGpdGpdApdApdApdApdGpdGpdCpdTpdGpdApdGpdApdGpdCpdTpdApdCpdGpdCp-3'
55 16990.09 g/mole 526900.00 L/(mole·cm) 49.09% 1.90 32.25

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Wang, L, et al. (2012). Selection of DNA aptamers that bind four organophosphorus pesticides. Biotechnol Lett, 2012(34), 869-874. doi: 10.1007/s10529-012-0850-6

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