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Apta-Index™: Phospholamban (Apt-9) (ID# 188)

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Phospholamban (Apt-9) (ID# 188)

Phospholamban (Apt-9)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Phospholamban Protein 20 nM (reported value) 10 mL of PBS (0..5% Tween 20) containing bead-bound PDZ-Myc-His6 (5 uL) and 1 ug/mL BSA was incubated for 30 minutes with library. N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-dTpdTpdGpdGpdGpdGpdApdGpdGpdGpdGpdCpdApdCpdTpdGpdGpdGpdCpdApdGpdTpdGpdTpdApdApdTpdTpdTpdApdCpdGpdApdApdApdGpdCpdGpdApdGp-3'
40 12546.21 g/mole 400500.00 L/(mole·cm) 55.00% 2.50 31.33

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Tanaka et al. "In Vitro Selection and Characterization of DNA Aptamers Specific for Phospholamban." The Journal of Pharmacology and Experimental Therapeutics, 329(2009): 57-63.

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