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Apta-Index™: Biotin (ID# 152)

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Biotin (ID# 152)

Biotin
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA Biotin Small Organic 5.7 µM (reported value) Selection Buffer (0.1 M KCl, 5 mM MgCl2, and 10 mM Na-HEPES (pH 7.4)) N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-rGprGprAprAprCprAprCprUprAprUprCprCprGprAprUprGprGprCprAprCprCprGprAprCprCprAprUprAprGprGprCprUprCprGprGprGprUprUprGprCprCprAprGprAprGprGprUprUprCprCprAprCprAprCprUprUprUprCprAprUprCprGprAprAprAprAprGprCprCprUprAprUprGprCprUprAprGprGprCprAprAprUprGprAprCprAprUprGprGprAprCprUprCprCprUprUprGprGprUprCprAprUprUprAprGprGprAprUprCprGp-3'
110 35326.27 g/mole 1080100.00 L/(mole·cm) 51.82% 0.93 32.71

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Wilson et al. "Functional Requirements for Specific Ligand Recognition by a Biotin-Binding RNA Pseudoknot." Journal of Biochemistry, 37(1998): 14410-14419.

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