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Apta-Index™: Sulforhodamine B (Clone 73 - min) (ID# 148)

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Sulforhodamine B (Clone 73 - min) (ID# 148)

Sulforhodamine B (Clone 73 - min)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Sulforhodamine B Small Organic 190 nM (reported value) Selection Buffer: 0.1 M KCl, 5 mM MgCl2, 10 mM Na-HEPES (pH 7.4). N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Kd value listed is for sulforhodamine B immobilized to a column. Free sulforhodamine B binds with Kd= 660 nM.
5'-dCpdCpdGpdGpdCpdCpdApdApdGpdGpdGpdTpdGpdGpdGpdApdGpdGpdGpdApdGpdGpdGpdGpdGpdCpdCpdGpdGp -3'
29 9155.94 g/mole 284400.00 L/(mole·cm) 82.76% 3.52 32.19

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Wilson C, Szostak J. (1998) Isolation of a fluorophore-specific DNA aptamer with redox activity. Chem Biol. 5(11):609-617.

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