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Apta-Index™: NF-kappa B (Aptamer 3) (ID# 141)

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NF-kappa B (Aptamer 3) (ID# 141)

NF-kappa B (Aptamer 3)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA NF-kappa B Protein 1 nM (reported value) Buffer A (20 mM HEPES (pH 7.5), 0.4 M NaCl, 2 mM EDTA, 5% v/v glycerol, 1 mM DTT, 0.5 mM PMSF, 1 ug/mL pepstatin, 1 ug/mL leupeptin) Buffer B (20 mM HEPES (pH 7.5), 2 mM EDTA, 5% v/v glycerol, 1 mM DTT, 0.5 mM PMSF, 1 ug/mL pepstatin, 1 ug/mL leupeptin) Selection Buffer (10 mM HEPES (pH 7.5) 0.1 M NaCl, 1 mM DTT) N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-rGprGprGprAprUprAprUprCprCprUprCprGprAprGprCprAprUprAprAprGprAprAprAprCprAprAprGprAprUprAprGprAprUprCprCprUprGprAprAprAprCprUprGprUprUprUprUprAprAprGprGprUprUprGprGprCprCprGprAprUprCprUprUprCprUprGprCprUprCprGprAprGprAprAprUprGprCprAprUprGprAprAprGprCprGprUprUprCprCprAprUprAprUprUprUprUprUp-3'
97 31122.71 g/mole 981700.00 L/(mole·cm) 41.24% 1.02 31.70

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Lebriska and Maher. "Selection and Characterization of an RNA Decoy for Transcription Factor NF-B." Journal of Biochemistry, 38(1999): 3168-3174.

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