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Apta-Index™: SARS Coronavirus NTPase/Helicase (ES15-1) (ID# 126)

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SARS Coronavirus NTPase/Helicase (ES15-1) (ID# 126)

SARS Coronavirus NTPase/Helicase (ES15-1)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA SARS Coronavirus NTPase/Helicase Protein 1.2 nM (reported value) Binding Buffer (30 mM Tris–HCl (pH 7.5), 150 mM NaCl, 1.5 mM MgCl2 , 2 mM dithiothreitol (DTT) and 1% (w/v) BSA) Termination Buffer (0.2 M EDTA, 0.2 M NaCl, and 20 mM HEPES (pH 7.4)) Buffer A (25 mM Tris–HCl (pH 7.5), and 0.3 mM NaCl) N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-rGprAprUprAprAprUprAprCprGprAprCprUprCprAprCprUprAprUprAprGprGprGprUprUprCprAprCprUprGprCprAprGprAprCprUprUprGprAprCprGprAprAprGprCprUprUprGprCprAprGprAprAprAprAprGprGprGprGprGprAprAprGprAprAprGprAprGprGprGprUprGprAprUprUprCprAprGprGprCprGprAprGprAprGprAprAprUprGprGprAprUprCprCprAprCprAprUprCprUprAprCprGprAprAprUprUprCp-3'
107 34719.10 g/mole 1104500.00 L/(mole·cm) 46.73% 0.91 31.43

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Jang, K., et al. "Isolation of inhibitory RNA aptamers against severe acute respiratory syndrome (SARS) coronavirus NTPase/Helicase." Biochemical and Biophysical Research Communications, 366(2008): 738-744.

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