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Apta-Index™: E. Coli Release Factor 1 (Clone II-1) (ID# 122)

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E. Coli Release Factor 1 (Clone II-1) (ID# 122)

E. Coli Release Factor 1 (Clone II-1)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA E. Coli Release Factor 1 Small Organic 30 nM (reported value) Elution Buffer (250 mM imidazole) N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Recombinant RF1 derived from e.coli (K-12 strain) was used to develop this aptamer.
5'-rGprGprAprCprCprGprAprGprAprAprGprUprUprAprCprCprCprUprGprUprAprAprUprCprUprUprAprGprGprAprUprGprAprAprUprCprGprCprAprUprGprCprUprCprUprAprGprCprGprAprCprCprUprUprUprUprCprGprGprCprUprUprCprGprGprCprGprUprAprCprGprCprAprCprAprUprCprGprCprAprGprCprAprAprCp-3'
85 27219.37 g/mole 824600.00 L/(mole·cm) 52.94% 1.21 33.01

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Sando, et al. "In vitro selection of RNA aptamer against Escherichia coli release factor 1." Bioorganic & Medicinal Chemistry Letters, 17(2007): 1216-1220.

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