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Apta-Index™: Interleukin 32 (IL-32) (AC3-3) (ID# 118)

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Interleukin 32 (IL-32) (AC3-3) (ID# 118)

Interleukin 32 (IL-32) (AC3-3)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA Interleukin-32 (IL-32) Protein 78 nM (reported value) Binding Buffer (20 mM sodium phosphate, 500 mM NaCl and 20 mM imidazole (pH 7.4))
Elution Buffer (20 mM sodium phosphate, 500 mM NaCl and 300 mM imidazole (pH 7.4)
At room temperature.
N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-rGprGprGprUprUprCprAprCprUprGprCprAprGprAprCprUprUprGprAprCprGprAprAprGprCprUprUprCprCprGprGprAprGprAprGprAprAprGprGprGprUprCprAprAprAprGprUprUprGprUprGprCprGprGprGprAprGprUprGprUprGprUprUprGprUprGprGprAprAprUprGprGprAprUprCprCprAprCprAprUprCprUprAprCprGprAprAprUprUprCp-3'
90 29155.58 g/mole 905300.00 L/(mole·cm) 51.11% 1.10 32.21

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Kim et al. "Generation of Antagonistic RNA Aptamers Specific to Proinflammatory Cytokine Interleukin-32." Bulletin of the Korean Chemical Society, 31 (2010): 3561-3566.

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