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Apta-Index™: D-Tryptophan (MF-10) (ID# 107)

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D-Tryptophan (MF-10) (ID# 107)

D-Tryptophan (MF-10)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA D-Tryptophan Small Organic 18 nM (reported value) Column Buffer (4 mM Mg2+, 250 mM NaCl, 50 mM Tris C1 (pH 7.6)) Binding Buffer is (250 mM NaCl, 4 mM MgCl2, 50 mM Tris (pH 7.6)) Elution Buffer (20 mM EDTA (pH 8.0)) N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-rAprGprUprAprAprUprAprCprGprAprCprUprCprAprCprUprAprUprAprGprGprGprAprGprAprAprUprUprCprCprGprAprCprCprAprGprAprAprGprUprUprGprGprCprGprUprUprGprGprCprAprUprGprAprCprGprCprGprGprGprGprAprAprUprCprGprGprGprUprGprCprAprUprCprGprAprUprGprAprCprUprAprCprUprCprCprUprGprGprGprCprCprCprAprCprGprUprCprUprGprUprUprGprUprUprGprAprCprGprUprCprAprCprAprGprCprUprUprGprAprUprUprUprAprGprGprAprUprAprGprCprGprCprUprUprGprGprGprCprAprGprUprCprGprUprGprCprAprGprUprGprGprAp-3'
153 49445.70 g/mole 1515800.00 L/(mole·cm) 53.59% 0.66 32.62

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Famulok and Szostak. "Stereospecific Recognition of Tryptophan Agarose by in Vitro Selected RNA." Journal of American Chemical Society, 114 (1992): 3990

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