Aptagen, LLC
RNA
cAMP
Small Organic
10 µM (reported value)
Binding Buffer (20 mM Tris-HCl (pH 7.5 at 23 °C), 450 mM NaCl, 100 mM KCl, 10 mM MgCl2, 1 mM MnCl2, and 5 mM CaCl2)
N/A°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
cAMP (ID# 104)
5'rGprGprAprAprGprAprGprAprUprGprGprCprGprAprCprUprAprAprAprAprCprGprAprCprUprUprGprUprCprGprCp3'
4
10044.17 g/mole
316000 L/(mole·cm)
51.61%
3.16
31.79
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Kozumi and Breaker. "Molecular Recognition of cAMP by an RNA Aptamer." Biochemistry, 39 (2000): 8983-8992.
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