Aptagen, LLC* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.(reported value) NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.