Aptagen, LLC
DNA
BoNT A Light Chain (BoNT/A-rLc)
Protein
34 nM (reported value)
1X Binding Buffer: 20 mM Hepes, 150 mM NaCl, 2 mM KCl, 2 mM MgCl2, 2mM CaCl2 (pH 7.4)
23°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Botulinum neurotoxin type A (BoNT A) Aptamer (ID# 600)
5'dCpdTpdTpdGpdApdGpdTpdGpdTpdCpdApdTpdGpdGpdApdCpdGpdTpdTpdCpdCpdGpdGpdTpdCpdTpdTpdGpdGpdGpdCpdGpdGpdGpdApdTpdApdTpdTpdTpdGpdTpdTpdTpdGpdTpdTpdTpdTpdCpdTpdGpdCpdCpdTpdApdTpdGpdTpdTp3'
60
18544.08 g/mole
547900 L/(mole·cm)
46.67%
1.83
33.85
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
(1) Bogomolova, A., Aldissi, M. ƒ??Real-time and label-free analyte detection in a flow-through mode using immobilized fluorescent aptamer/quantum dots molecular switches.ƒ? Biosensors and Bioelecgtronics, 66 (2015): 290-296. (2) Lou, X., et al. ƒ??Micromagnetic selection of aptamers in microfluidic channels.ƒ? PNAS, 106, 9 (2009): 2989-2994.
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