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Aptamer Details

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RNA Tobramycin Molecular Beacon (BA 14-2) (ID# 45)

RNA Tobramycin Molecular Beacon (BA 14-2)
RNA Tobramycin Small Organic 16 µM (reported value) Binding buffer: 50 mM Tris (pH 7.4), 500 mM NaCl NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'rGprGprAprAprUprGprGprAprUprCprCprAprCprAprUprCprUprAprCprGprAprAprGprGprCprUprUprUprGprAprAprGprGprUprGprAprGprAprCprCprGprUprGprCprAprAprAprUprGprAprGprGprAprUprGprGprUprGprUprGprGprAprUprGprAprUprUprAprGprGprGprUprUprGprUprCprGprGprUprUprUprUprCprAprCprUprGprCprAprGprAprCprUprUprGprAprCprGprAprAprGprCprUprUp3' 104 33690.28 g/mole 1052600 L/(mole·cm) 48.08% 0.95 32.01

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Morse, Daniel. "Direct selection of RNA beacon aptamers." Biochemical and Biophysical Research Communications, 359 (2007): 94ƒ??101.

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